Some foreign DNA fragments are attached to cla I site of pBR322. This recombinant vector is used to transform E. coli host cells. The cells subjected to transformation are plated on two different media, one containing ampicillin and other containing tetracycline. The transformed cells containing the recombinant vector :
a.Will grow on both tetracycline containing and ampicillin containing media
b.Will not grow on either tetracycline containing or ampicillin containing media
c.Will grow on tetracycline, but not on ampicillin containing medium
d.Will grow on ampicillin, but not on tetracycline containing medium
Answers
Some foreign DNA fragments are attached to cla I site of pBR322. This recombinant vector is used to transform E. coli host cells. The cells subjected to transformation are plated on two different media, one containing ampicillin and other containing tetracycline. The transformed cells containing the recombinant vector (a)Will grow on both tetracycline containing and ampicillin containing media
Explanation:
1.pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after Francisco Bolivar Zapata, the postdoctoral researcher and Raymond L. Rodriguez.
2.pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Plasmid pBR322 confers resistance to ampicillin and tetracycline. Since, the foreign DNA fragment is attached to Cla 1 site, the transformant will be observed on both the plates.