Question

southern blotting technique involves the transfer of DNA from a)gel to memberance b)membrance to cell c)solution to gel d) gel to solution

Answer 1

Answer:

The correct option is(a).

Explanation:

Southern blotting is defined as transfer of DNA fragments from the agarose gel to nitrocellulose membrane .This technique was discovered by E.M. Southern. And this technique is widely used in molecular biology to identify the DNA fragments and also used to check for the presence of a specific DNA sequence in a DNA sample.

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Answer 2

Answer:

The correct option is a)gel to memberance.

Explanation:

• The transfer of DNA from agarose gels to membranes for composition analysis is known as southern blotting. The first step is to isolate the DNA from the sample's cell, which was taken from the crime scene.
• Next, the DNA is digested with a restriction enzyme and the fragments are separated using gel electrophoresis. DNA is then transferred to a synthetic membrane after that.
• The DNA fragments continue to be separated in the same manner as on the gel. The blot is then incubated with several copies of a probe, which base pairs with its complementary DNA sequence to create a double-stranded DNA molecule.
• Normally radioactive, the probe is rendered visible by X-ray exposure.

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