state source of errors in estimation of total wbc count
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Explanation:
Errors in counting total RBCs and WBCs by haemocytometer: Errors in dilution. ... Inadequate or improper shaking of the pipette after dilution. Failure to wipe excess blood from the end of the pipette.
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The hemocytometer continues to be the industry standard for counting laboratory cells. The hemocytometer was first used in the 18th century in France to evaluate patient blood samples. Over the previous several centuries, it has undergone a number of significant changes, resulting in a contemporary instrument that is more precise and user-friendly than its forebears.
Explanation:
- Human mistake (mixing, handling, dilution, miscalculation, and procedural errors made by humans)
- To achieve statistical correctness, numerous cell sample counts are required.
- homogeneous cell distribution is necessary
- Variations in instruments and materials (grids, depth, coverslips, type of buffer, and pipettes)
- Today's cell counting is more accurate and efficient thanks to the use of contemporary automation, which has essentially removed many of these sources of error.
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