Biology, asked by faisaljbrn2390, 1 year ago

Steps involved in preparation of temporary mount of onion peel cells

Answers

Answered by Raju2392
1
Aim
To prepare a stained, temporary mount of onion peel and to study its cells.

Theory
All living organisms are composed of cells. New cells arise by the division of pre-existing cells. Cell is the structural and functional unit of life. In plants, cells have an outermost rigid cell wall beneath which is a cell membrane. The cell membrane encloses cytoplasm, cell organelles, and a nucleus.

Materials Required
An onion bulb, slides, cover slips, two watch glasses, needle, brush, forceps, razor blade, compound microscope, blotting paper, methylene blue solution (or safranin), glycerine, and water.

Procedure
Step 1: One fleshy scale leaf of an onion is taken. It is broken into two and a thin membranous peel is pulled out using a forcep adhering to the inner surface of the leaf. This is the epidermal peel.
Step 2: The peel is placed in a watch glass containing water and is cut into small rectangular pieces.
Step 3: 1 or 2 drops of methylene blue or safrarin is mixed in a small quantity of water taken in another watch glass. The peel is transfered into it. Peels are leave for about 3 minutes. The peel is dipped in water to remove excess stain.
Answered by gamingbrownie11
0

Answer:

Aim

To prepare a stained, temporary mount of onion peel and to study its cells.

Theory

All living organisms are composed of cells. New cells arise by the division of pre-existing cells. Cell is the structural and functional unit of life. In plants, cells have an outermost rigid cell wall beneath which is a cell membrane. The cell membrane encloses cytoplasm, cell organelles, and a nucleus.

Materials Required

An onion bulb, slides, cover slips, two watch glasses, needle, brush, forceps, razor blade, compound microscope, blotting paper, methylene blue solution (or safranin), glycerine, and water.

Procedure

Step 1: One fleshy scale leaf of an onion is taken. It is broken into two and a thin membranous peel is pulled out using a forcep adhering to the inner surface of the leaf. This is the epidermal peel.

Step 2: The peel is placed in a watch glass containing water and is cut into small rectangular pieces.

Step 3: 1 or 2 drops of methylene blue or safrarin is mixed in a small quantity of water taken in another watch glass. The peel is transfered into it. Peels are leave for about 3 minutes. The peel is dipped in water to remove excess stain.

Explanation:

All living organisms are composed of cells. New cells arise by the division of pre-existing cells. Cell is the structural and functional unit of life. In plants, cells have an outermost rigid cell wall beneath which is a cell membrane. The cell membrane encloses cytoplasm, cell organelles, and a nucleus.

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