Steps involved in preparation of temporary mount of onion peel cells
Answers
To prepare a stained, temporary mount of onion peel and to study its cells.
Theory
All living organisms are composed of cells. New cells arise by the division of pre-existing cells. Cell is the structural and functional unit of life. In plants, cells have an outermost rigid cell wall beneath which is a cell membrane. The cell membrane encloses cytoplasm, cell organelles, and a nucleus.
Materials Required
An onion bulb, slides, cover slips, two watch glasses, needle, brush, forceps, razor blade, compound microscope, blotting paper, methylene blue solution (or safranin), glycerine, and water.
Procedure
Step 1: One fleshy scale leaf of an onion is taken. It is broken into two and a thin membranous peel is pulled out using a forcep adhering to the inner surface of the leaf. This is the epidermal peel.
Step 2: The peel is placed in a watch glass containing water and is cut into small rectangular pieces.
Step 3: 1 or 2 drops of methylene blue or safrarin is mixed in a small quantity of water taken in another watch glass. The peel is transfered into it. Peels are leave for about 3 minutes. The peel is dipped in water to remove excess stain.
Answer:
Aim
To prepare a stained, temporary mount of onion peel and to study its cells.
Theory
All living organisms are composed of cells. New cells arise by the division of pre-existing cells. Cell is the structural and functional unit of life. In plants, cells have an outermost rigid cell wall beneath which is a cell membrane. The cell membrane encloses cytoplasm, cell organelles, and a nucleus.
Materials Required
An onion bulb, slides, cover slips, two watch glasses, needle, brush, forceps, razor blade, compound microscope, blotting paper, methylene blue solution (or safranin), glycerine, and water.
Procedure
Step 1: One fleshy scale leaf of an onion is taken. It is broken into two and a thin membranous peel is pulled out using a forcep adhering to the inner surface of the leaf. This is the epidermal peel.
Step 2: The peel is placed in a watch glass containing water and is cut into small rectangular pieces.
Step 3: 1 or 2 drops of methylene blue or safrarin is mixed in a small quantity of water taken in another watch glass. The peel is transfered into it. Peels are leave for about 3 minutes. The peel is dipped in water to remove excess stain.
Explanation:
All living organisms are composed of cells. New cells arise by the division of pre-existing cells. Cell is the structural and functional unit of life. In plants, cells have an outermost rigid cell wall beneath which is a cell membrane. The cell membrane encloses cytoplasm, cell organelles, and a nucleus.