Question

Structure of an archaeal bfamily dna polymerase in complex with a chemically modified nucleotide

Answer 1

Archaeal B‐family DNA polymerases (DNA pols) are the driving force of cutting edge biotechnological applications like next‐generation sequencing. The acceptance of chemically modified nucleotides by the DNA pols is key to these technologies.

Answer 2

Answer:

We also crystalized the A-family DNA pol KlenTaq with the same nucleotide. The reported structural data reveal how the protein and the DNA modulate two distinct conformations of the appended moiety in the A- and B-family DNA pols and how these influence the processing of the modified nucleotide