Question

Suggest reasons for the higher activity of immobilized sucrase over the range of pH between 5.5 and 8.0.

Answer 1

Answer:

Maybe it has to do with the the fact that chitosan has many primary amino groups. At the lower pH, these will nearly all be protonated, giving the particle a high positive charge. The charge may repel the substrate of the enzyme from the particle, or adversely affect the function of the enzyme. At the higher pH, the positive charge of the particle will be lower because of deprotonation of the amino groups.

Explanation:

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