Question

Summarise the process by which the sequence of DNA bases in Human Genome Project was determined using the method developed by Frederick Sanger. Name of free-living non-pathogenic nematode whose DNA has been completely sequenced​

Answer 1

Explanation:

Sanger was awarded the Nobel Prize in Chemistry once again in 1980, this time sharing it with Paul Berg and Walter Gilbert for determining the amino acid sequences of DNA information.

Answer 2

Frederick Sanger Method.

Explanation:

• The process is Sanger sequencing which is also known as the Chain Termination Method.

• This method was discovered in 1977 by Frederick Sanger and colleagues.
• It is a selective incorporation of dideoxynucleotides or ddNTPs by enzyme DNA polymerase.

• Sanger sequencing method was used to determine the sequences of small fragments of human DNA in human genome project.
• Caenorhabditis elegans is a namatode which is free living, non pathogenic, whose DNA is completely sequenced.