Summer portfolio work for dass 7ABC SST
Topic Object or artefact( To focus interest)
I. Study the given artefact or object belonging to another culture or group from the past and
answer the following questions,
1 what are your first impressions of this object
2. What appears to be the main purpose of this object?
Answers
Answer:
I will be cryo-fixing a variety of plant species (woody eudicots) for ultrastructure studies via transmission electron microscopy. Previously, I have used standard chemical fixation techniques, which have not provided optimal morphology preservation. Given that cryo-fixation may be better suited for preservation and eliminating artifacts, I am interested in maximizing leaf sucrose content (sucrose serves as a cryo-protectant) in order to minimize crystal formation.
In the past, my lab has left plants in our dark room overnight (16 hours) to allow for starch degradation. My thought is that degradation of starch for chemical fixation is ideal, as starch decreases fixation penetration efficiency and time. In the context of cryo-fixing, I would guess that maximizing starch content is ideal as this will allow for faster freezing.
Any thoughts or suggestions on minimizing artifacts with cryo-fixing plant tissues would be greatly appreciated!
Explanation:
Hope it helps you
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