Question

Suppose generation time of a bacterium is 90 minutes and initiao number of a cell culture is 1000 cell at the start of log phase. how many bacteria will be there be after 8 hours nof exponential growth

Answer 1

Population doubles: generation time

Mean generation time = mean time of doubling

Let the initial population number

             the population at time 

             the number of generations in time 



, and



k = mean growth rate constant (the number of generations per unit time)



g = mean generation time (mean time per doubling)



 



 





 

 

Problem: If we begin with 109 bacteria (1 mg wet weight), and the bacteria double every 30 minutes, what will the wet weight of bacteria be after 24 hours?

How might you use this?

 

ï Your boss has already told you that she needs 100 g dry weight of an E. coliculture, and asked you to calculate how many liters of culture you will have to grow, assuming that you want to harvest the cells at a cell density of 109cells/ml.

            Now she tells you that you will be doing this routinely, and she wants you to be able to inoculate cultures at 5:00pm, just before you leave, and have them ready, still growing exponentially, the next morning when you start at 8:00 am. How many cells do you need 





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