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sai sampath
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i will really give ponts for all
and foolow for futu re e questions i will slowly inc re ease points fo re each question
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Explanation:
Experience in immunology investigating the role of various immune cells like monocyte- macrophages, microglia involved in neurodegeneration using Biochemical, Pathological and molecular techniques.
• Ph.D. in pharmaceutical sciences
• Abilities in project management and in the resolution of problems
• Autonomous and proactive
• Ease to work within a team environment
• good communication skills
Published work in various peer-reviewed journals.
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Experience
Amorchem therapeutics( cervo brain research center)
Research Scientist
Amorchem therapeutics( cervo brain research center)
Nov 2018 – Present1 year 9 months
Quebec, Quebec, Canada
1. SRSF3, as a key regulator of RNA splicing/transport and gene expression, which appears to play a central role analogous to a checkpoint of the innate immune system. As for T-cell checkpoints in oncology, removal of this off-switch in the innate immune response is predicted to have beneficial effects in human CNS disease, and particularly in diseases with an inflammatory component. The group is examining multiple modalities (anti-sense, siRNA, mAb) of SRSF3 targeting, and determining their...
1. SRSF3, as a key regulator of RNA splicing/transport and gene expression, which appears to play a central role analogous to a checkpoint of the innate immune system. As for T-cell checkpoints in oncology, removal of this off-switch in the innate immune response is predicted to have beneficial effects in human CNS disease, and particularly in diseases with an inflammatory component. The group is examining multiple modalities (anti-sense, siRNA, mAb) of SRSF3 targeting, and determining their usefulness in animal modeling of frontotemporal dementia and ALS.
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CRIUSMQ
Doctoral Student
CRIUSMQ
Sep 2012 – Oct 20186 years 2 months
Quebec, Canada
- Conception, leading and execution of various research projects
- Writing manuscript
- Scientific communication (oral presentations and posters in national and international meetings
Technical skills:
Molecular Biology: Polymerase chain reaction, Gel electrophoresis, protein analysis by immuno blot and Immunofluorescence, Plasmid extraction, qPCR, Proteomics and Transcriptomics, Protein-protein interactions by Immunoprecipitation, Cell based assays like MTT assay,...
- Conception, leading and execution of various research projects
- Writing manuscript
- Scientific communication (oral presentations and posters in national and international meetings
Technical skills:
Molecular Biology: Polymerase chain reaction, Gel electrophoresis, protein analysis by immuno blot and Immunofluorescence, Plasmid extraction, qPCR, Proteomics and Transcriptomics, Protein-protein interactions by Immunoprecipitation, Cell based assays like MTT assay, Phagocytosis assay, ELISA.
Animal handling: Rat and mice handling, dosing, necropsy, tissue collection and genotyping.
Imaging: in vivo bio-luminescent imaging using the IVIS 100 and luciferase assays, photon measurements using living image software.
Cell biology: Culturing and sub-culturing adherent and suspension cell lines, Primary microglial culture, human Macrophage/ Monocyte culture, immortalized human cancer cell lines, transfection, cell counting, cell sorting by multi-color flow cytometry analysis.