Question

The A260 of a plasmid solution after 100-fold dilution is 0.2. Given that A260 of 1.0

represents 50 µg/ml of DNA and the total volume of isolated plasmid solution is 50 µl,

what will be the concentration and amount respectively of the isolated plasmid?​

Answer 1

kon se class Ka h

Explanation:

mujhe to nhi aa rha h

Answer 2

Answer:

1 ug/ul and 50ug respectively.

Explanation:

A260 of 1 corresponds to 50ug

thus 0.2 would correspond to 10ug

therefore 10ug/ml

but this concentration is for 100 fold dilution

therefore original concentration 10ug/10ul = 1ug/ul

amount = 50ug as 1ul will have 1ug, so 50ul will have 50ug

hope its clear