Question

The advantages of suspension culture over adherent culture

Answer 1

Appropriate for cells adapted to suspension culture and a few other cell lines that are nonadhesive (e.g., hematopoietic)  

Easier to passage, but requires daily cell counts and viability determination to follow growth patterns; culture can be diluted to stimulate growth

Does not require enzymatic or mechanical dissociation

Can be maintained in culture vessels that are not tissue-culture treated, but requires agitation (i.e., shaking or stirring) for adequate gas exchange