Question

The bond digested by Hind II is

Hydrogen bond between adjacent nucleotides on same strand

Hydrogen bond between adjacent nucleotides on different strands

Phosphodiester bond between adjacent nucleotides on same strand

Bond between sugar and nitrogenous base of adjacent nucleotides on same strand​

Answer 1

Answer:

All Nucleotides have a common structure. (a) Chemical structure of adenosine 5′-monophosphate (AMP), a nucleotide that is present in RNA. All nucleotides are composed of a phosphate moiety, containing up to three phosphate groups, linked to the (more...)

The base components of nucleic acids are heterocyclic compounds with the rings containing nitrogen and carbon. Adenine and guanine are purines, which contain a pair of fused rings; cytosine, thymine, and uracil are pyrimidines, which contain a single ring (Figure 4-2). The acidic character of nucleotides is due to the presence of phosphate, which dissociates at the pH found inside cells, freeing hydrogen ions and leaving the phosphate negatively charged (see Figure 2-22). Because these charges attract proteins, most nucleic acids in cells are associated with proteins. In nucleotides, the 1′ carbon atom of the sugar (ribose or deoxyribose) is attached to the nitrogen at position 9 of a purine (N9) or at position 1 of a pyrimidine (N1).

Answer 2

The bond digested by Hind II is a Hydrogen bond between adjacent nucleotides on different strands. (OPTION B)

HIND II RESTRICTION ENZYME -

• The enzymes cleave the DNA at a specific recognition site producing blunt ends.
• The recognition site of the enzyme is GTPyPuAC between unspecified pyrimidine and purine.
• It makes cut after Y-3 at the site of DNA.
• The first letter is from the genus, the second and third letter from the species, and the fourth letter from the bacterial strain.
• HIND II is derived from Haemophilus Influenza Rd and the strain is second from the bacteria.