Question

The cell disruption is important step in isolation of extracellular enzyme.(True/False).

Answer 1

Cell disruption is an essential initial preparative step for the purification of intracellular protein products. Different methods of cell disruption have been reviewed by several researchers7 particularly focusing on large-scale operations.8 Different methods of cell disruption that are currently available can be conveniently divided into two main groups: (i) mechanical; and (ii) non-mechanical. Complete destruction of the cell wall in a non-specific manner is usually achieved by mechanical means exploiting solid-shear (bead mill) and liquid-shear forces (high-pressure homogeniser or micro-fluidiser). Non-mechanical methods are judged to be more benign and often only perforate or permeabilise cells rather than tearing them apart. For example, chemical and enzymatic methods rely on selective interaction of a substance, or an enzyme, respectively with components of the cell wall or the membrane which modify the cell boundary and allow product to seep out.