Question

The correct sequence of making a cell competent is

Answer 1

The correct sequence of making a cell competent is. incubation of cells with recombinant DNA on ice → heat shock (42∘C)→treatment with divalent cations → placing on ice.

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Answer 2

Answer:

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explanation is below...

inorder to force a bacteria to take up the plasmid the bacterial cell must to be made competent.this is done by treating them with a specific concentration of divalent cations such as calcium which increases the efficiency with which the dna molecule enters into the bacteria through pores in it cell wall.recombinant dna then forced into such cell by incubating the cells with recombinant dna on ice followed by placing them immediately at heat shock and then again into ice.this enable the bacteria to take up the recombinant dna