the function of ligase is
Answers
Answer:
Ligase also called as ‘Biological Glue’ helps to link the cut desirable piece of DNA to the vector DNA.
Answer:
In particular, Okazaki fragments and the primary DNA piece are joined by DNA ligase.
Explanation:
During DNA replication, DNA ligase is used to "glue" DNA fragments together to create new DNA strands. By creating phosphodiester linkages between DNA monomers, it does this. A fundamental class of enzymes known as DNA ligases is required for fusing DNA strands together by facilitating the formation of the phosphodiester bond. By repairing breaches in the phosphodiester links of DNA that happen during replication and recombination, DNA ligase regulates DNA integrity. Note that DNA ligases can only connect two nucleotides by creating phosphodiester linkages. The double-stranded DNA is unzipped. Any cracks in the sugar-phosphate backbone are sealed by it.
Thus, in recombinant technology, DNA ligase is used to separate DNA fragments according to their length and electrical charges.