the methodology which has been most useful for investigating the calvin cycle is
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Calvin, Benson and Bassham utilised C14 (with long life) to trace the path of carbon in photosynthesis. Calvin was awarded Nobel Prize in 1961 in recognition to his work with Cl4 isotope. He discovered the cycle involved in carbon assimilation, known as Calvin cycle or C3-cycle.
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Radioactive isotope technique has helped in the investigation of Calvin cycle. Melvin Calvin used radioactive 14C in algal photosynthetic studies. With the help of 14C, he discovered that PGA is the first C O 2 CO_{2} CO2 fixation product in photosynthesis of alga
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