Question

The most important feature in a plasmid to serve as a vector in Gene cloning experiment is
A) Ori
B) Selectable marker
C) Size
D) Site for restriction endonuclease

Answer 1

Answer: (A)Ori

Vectors are used to deliver the desired foreign DNA into a host cell.

Ori (Origin of replication )is a sequence from where the replication starts.

Hence any piece of DNA when linked to this sequence can be made to replicate in the host cell.

It is a vector whose origin supports a high copy number ,more numbers of copies of the target DNA that would be cloned.