The onion peel should neither be stained too dark nor too light in the process of making slides
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When the peel of onion is kept in a drop of water (isotonic solution), there is no observable change in cells.
When the peel of onion is kept in concentrated solution (hypertonic), the protoplasm shrinks as the water starts moving out due to exosmosis.
When the peel of onion is kept in concentrated solution (hypertonic), the protoplasm shrinks as the water starts moving out due to exosmosis.
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