Question

the procedure to the activit you perfomed in your school to observe pollen grain of flowers​

Answer 1

Answer:

Full SiteNavigation

Pollen Under The Microscope

Methods, Techniques and Observations

What is Pollen?

Pollen is a small grain that consists of a few cells. To the naked eye it appears as a yellowish (pale yellow) dust-like substance that is either dispersed by wind or insects.

Pollen is formed within the sacs (or microsporangia) in the anthers that are located in plant flowers. The development of plant anthers also involves the growth and differentiation of tissue that develop to produce the pollen sacs. This is then followed by a process of cell division (meiosis) that results in the formation of clusters referred to as quartets within the pollen sac chambers.

The quartet then undergoes further divisions to produce pollen, which serves as the male gametophyte of plants (seed plants) for reproduction. Depending on the type of plant, pollen is either dispersed by wind or insect to the receptive stigma.

* A plant that is pollinated through wind is referred to as anemophilous while those by insects are known as entimophilous.

* Once a pollen grain falls on the receptive stigma, it starts absorbing water and becomes activated. This causes the gametophyte in the grain to develop a tube reaching the ovule of the flower, which in turn allows male gamete cells to be transported to the ovule for fertilization.

Pollen Microscopy

Sample Collection

Requirements

Needle (or wire loop)

Pipette tips

Tweezers

Procedure

Collect healthy anthers from flowers (this may involve inspecting a number of flowers to find healthy anthers with high pollen content)

Using the needle or laboratory wire loop, carefully and gently try scraping the pollen from the anthers and store them in the pipette tip or a micro-tube.

* If the pollen is not to be used immediately, it should dried and stored frozen.