the process of osmosis by patoto experiment
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Materials Required
Real Lab Procedure
Place the peeled potato on the tile and using the knife, cut both ends of the potato to make it flat.
Use the knife to make a cavity at the centre of the potato from one of the flat sides almost up to the bottom.
Pour distilled water into the Petri dish until it is half full.
Now, place the potato in the Petri dish.
Fill half the cavity made in the potato with 20% sugar solution.
Mark the level of sugar solution in the cavity using a pin.
The potato now functions as an osmometer.
Leave the osmometer undisturbed for about two hours.
Mark the rise in the level of the sugar solution in the cavity with another pin.
Simulator Procedure (as performed through the Online Labs)
To increase or decrease the concentration of the sugar solution in the Petri dish, choose the concentration of sugar from the 'In Petri dish: Select the sugar concentration’ drop down list.
To increase or decrease the concentration of the sugar solution in the potato, choose the concentration of sugar from the ‘In potato: Select the sugar concentration’ drop down list.
Click on the Check box ‘Cross sectional view’ to view the cross section of the potato, that helps view the water level inside potato.
Drag a pin to mark the initial level of the sugar solution in the cavity.
Click on the start button to start the experiment.
A timer is shown and you need to wait till the timer stops.
Drag another pin and mark the rise in level of the sugar solution in the cavity.
Click on the information icon to see the inference.
You can redo the experiment by clicking on the ‘Reset’ button.
Observation
The level of sugar solution in the potato cavity rises after some time due to the entry of water into the sugar solution through the selectively permeable membrane of the cells of the potato.
Conclusion
The movement of water from the Petri dish to the potato cavity occurs because of the difference in the concentration of solvent molecules in the two regions: sugar solution in the potato cavity and pure water in the Petri dish.
Precautions
The cavity should be deep enough to keep only a thin layer of tissues at the base of the potato.
The sugar solution should be of sufficiently high osmotic concentration as compared to the cell sap of potato cells.
Materials Required
Real Lab Procedure
Place the peeled potato on the tile and using the knife, cut both ends of the potato to make it flat.
Use the knife to make a cavity at the centre of the potato from one of the flat sides almost up to the bottom.
Pour distilled water into the Petri dish until it is half full.
Now, place the potato in the Petri dish.
Fill half the cavity made in the potato with 20% sugar solution.
Mark the level of sugar solution in the cavity using a pin.
The potato now functions as an osmometer.
Leave the osmometer undisturbed for about two hours.
Mark the rise in the level of the sugar solution in the cavity with another pin.
Simulator Procedure (as performed through the Online Labs)
To increase or decrease the concentration of the sugar solution in the Petri dish, choose the concentration of sugar from the 'In Petri dish: Select the sugar concentration’ drop down list.
To increase or decrease the concentration of the sugar solution in the potato, choose the concentration of sugar from the ‘In potato: Select the sugar concentration’ drop down list.
Click on the Check box ‘Cross sectional view’ to view the cross section of the potato, that helps view the water level inside potato.
Drag a pin to mark the initial level of the sugar solution in the cavity.
Click on the start button to start the experiment.
A timer is shown and you need to wait till the timer stops.
Drag another pin and mark the rise in level of the sugar solution in the cavity.
Click on the information icon to see the inference.
You can redo the experiment by clicking on the ‘Reset’ button.
Observation
The level of sugar solution in the potato cavity rises after some time due to the entry of water into the sugar solution through the selectively permeable membrane of the cells of the potato.
Conclusion
The movement of water from the Petri dish to the potato cavity occurs because of the difference in the concentration of solvent molecules in the two regions: sugar solution in the potato cavity and pure water in the Petri dish.
Precautions
The cavity should be deep enough to keep only a thin layer of tissues at the base of the potato.
The sugar solution should be of sufficiently high osmotic concentration as compared to the cell sap of potato cells.
Answered by
8
Cut a potato in two, and immerse one of the halves in a very salty solution of water — one containing a quarter cup of salt in a cup of water. Immerse the other piece in tap water containing no added salt. Leave both in their respective solutions for half an hour, then remove the potato halves from their solutions and observe their differences. The one in the salty solution will have shrunk, indicating that water is diffusing from a less concentrated solution to a more concentrated solution. The one in the tap water solution, in contrast, will actually swell slightly, indicating that it is taking in water.
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