The propase i stage of meiosis plays a vital role in r-dna formation.Justify with answer.
Answers
Meiosis
An unusual type of cell division produces two haploid cells in the first part of meiosis (I
Adding chromosome (a process called synapsis) is known as either a biennial (two chromosomes) or a tetrahed (four chromatids) to form a structure.
Prophase is the platform of Meiosis where homologous chromosome pair and exchange DNA (genetic re-evaluation).
Recombinant DNA Technology (Review)
In modern biology, genetic re-evaluation is an applied procedure.
In recombinant DNA technology (slang = genetic engineering), many techniques are used to replicate and make specific pieces of DNA in "large" amounts.
Recombinant DNA molecules are produced by...
1) Ban DNA from two different sources with different endoclies (restriction enzyme)
2) Mixing pieces together to allow intervals to be interrupted
3) Adding the pieces with DNA ligases.
Cloning specific DNA pieces are usually included:
1) DNA insertion in a vector (a recombinant vector)
2) Introduction to the recombinant vector in cells (usually E. coli)
3) Amplification of recombinant vector in cells
4) Selection of cells which take recombinant vectors.
5) Identification of the correct recombinant clone.
The "shotgun" approach is often used to create a clone.
This means that instead of starting with a known special section of DNA, resulting in a source of "source" DNA from a source (as relatively random pieces are cloned in a vector) in a library of clones.
If the source of DNA is the genome of the organism, then the library is presented as a genomic library.
To examine the genes of the organism, the use of the mRNA enzyme reverse transcripts can be "converted" into a complementary DNA (CDNA) library.
CDNA Poly-T primers are made by annealing in separate MRNA poly-a-tail and synthesized SSDNA with reverse transcripts from the MRNA template.
RNA is hydrolyzed and DNA polymerase produces the second strand to make the DSDNA.
The cDNA is then inserted into the vector and is propagated in the form of the above.
As the technique improves, large parts of DNA can be cloned as continuous pieces in specialized vectors such as the universe and yeast artificial chromosomes (YAC).