Question

To amplify one DNA sequence of interest,
how many types of primers are required
(A)
One
(B) Two
(C) Three
(D) Four

Answer 1

Answer:

two

Explanation:

The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have 2^n (2 to the n:th power) copies of DNA. For example, after 10 cycles you have 1024 copies, after 20 cycles you have about one million copies, etc.

Answer 2

To amplify one DNA sequence of interest, two types of primers are required.

They are forward primers and reverse primers.

• For amplifying a DNA sequence of interest, two sets of primers are required to copy or replicate the two strands of DNA.

• The two primers are meant to be complementary to the start and end of the DNA section that needs to be amplified.

• The forward primer binds to the start codon of antisense or template DNA whereas the reverse primer binds to the complementary strand of the sense DNA.