To detect the Transfection which gene is cotransfected along with transgene?
Answers
Answer:
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Explanation:
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Answer:
To detect transfection, a reporter gene is co-transfected along with the transgene.
Explanation:
A reporter gene is usually a tagged protein that fluoresces inside the cell. When transfected along with the transgene, the reporter gene serve as a detection control to determine the quality, or percentage of transfection. The reporter protein can be detected in a cell about 24 hours after transfection.
The number of cells showing the fluorescent protein as opposed to the total number of cells gives the proportion of transfection. Examples of reporter proteins are: green fluorescent protein, red fluorescent protein.