To extract dna from cells, what must you isolate it from in the case if a plant such as kiwi
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APSEducationK-12Lessons and LaboratoriesClassroom Activities in Plant BiotechnologyActivity 1 - DNA Extraction
Activities: Classroom Activities in Plant Biotechnology
Activity 1 - DNA Extraction
We will extract DNA from fruit to investigate how it looks and feels. This procedure is similar to what scientists have to do before they can use the information contained in this DNA. This information can be used to improve crops so that they are more resistant to disease, insect invasion or changes in climate.
Figure 1
Figure 2
Objectives
Extract DNA from plant cells
Understand the general structure of cells
Teacher preparation for experiment
Time Required: ~ 20 minutes
*Night before put 95% ethanol in freezer*
Make extraction solution (see below).
Start water heating to 60°C.
Prepare ice-water bath.
Prepare fruit pieces.
Gather materials for each student group as listed below.
Extraction Solution
Materials (100 ml)
10 ml of clear shampoo (Suave daily clarifying shampoo)
1.5 g of table salt
Distilled H2O
Procedure (modify amount depending on the size of a class)
Mix 90 ml of distilled water and 1.5 g of salt.
Add shampoo until solution volume is 100 ml. Stir slowly to avoid foaming of the shampoo.
Measure 20 ml of solution into 1L zipper bags (1 per student pair).
Student Activity - DNA Extraction
Materials
Time required: ~ 45 minutes
1-liter Zipper bag (one per student pair) with 20 ml of extraction buffer
Skinned and freshly cut kiwi fruit (each fruit cut into 12 pieces) or one large strawberry (each provides ~30 g per student pair)
500 ml beaker (class)
Hot water plate with beaker or saucepan of water set at a constant 60°C (class)
Cheese cloth (cut to fit over small beaker)
Tape
Large cooler with ice water bath (class)
Ice cold 95% ethanol (2 ml per student pair)
1 small test tube (1 per student pair)
1 wood applicator (1 per student pair)
Transfer pipettes