to study stomata by preparing a temporary amount of leaf peel
Answers
Answer:
Stomata are tiny openings or pores which are commonly noticed being distributed on the epidermis of the leaves and also in young stems. Typically, stomata are found on the lower surface of a dicot leaf and in a monocot leaf, on both of its surfaces. Stomata carry out the function of regulating gas exchange and water vapour between the leaves of the plant and the atmosphere.
Aim
To prepare a temporary mount of a leaf peel in order to show the stomata of a leaf
Principle/Theory
Plants are the primary producers. They carry out physiological processes such as photosynthesis and respiration which requires a gas exchange between the tissues of plants and the atmosphere. This process is carried out through tiny openings located in leaves, known as stomata.
See Also: Difference Between Photosynthesis and Cellular Respiration
Describe the structure of stomata.
Stomata are small elliptical openings on leaves that contain chloroplasts. They are girdled by two-kidney shaped cells known as guard cells on either side of the stomata. The guard cells possess a thick inner wall and a thin outer covering which control the closing and opening of the pores of stomata.
Discuss the closing and opening of the stomata.
Turgidity of the guard cells causes the stomata to open while the flaccid nature of the guard cells causes the stomata to close.
Material Required
A potted plant of Bryophyllum or Tradescantia
Needles
Forceps
Watch glass
Dropper
Glass slides
A brush
Coverslips
Blotting paper
Safranin
Compound microscope
Glycerine
Procedure
Pick a healthy leaf from the potted plant
Fold the leaf to gently pull the peel apart to separate a peeled section from the lower surface of the leaf. Use the forceps to perform this step. Allow the peel to remain in a watch glass holding water for some time.
In the watch glass, stain the sample by adding some drops of safranin through a dropper.
Take the peel out after 2-3 minutes. Set it on a clear glass slide
Add a drop of glycerin on the peel. Put a clear coverslip over it gently using a needle.
Excess glycerin and stain can be removed using blotting paper
Examine the slide first under a low-power and then under a high-power magnification of a compound microscope.
Diagram
Stomata opening and closure
Observation
Visible epidermal cells. The cells in their outline are irregular with no intercellular spaces
Small openings, stomata are scattered through the epidermal cells
Guard cells are observed which have chloroplasts and nucleus
Guard cells are observed having a thin outer covering and a thick inner boundary(concave)
Guard cells control the closing and opening of the stomata.
Conclusions
Epidermal cells are found containing stomata on the lower surfaces of the leaf.
Precautions
Avoid folding the leaf too much. The peel should be snipped to a proper size
The peel should always be placed at the centre of the slide and the slides should be held from the sides.
The peel should neither be overstrained nor under strained
A brush should be used to handle the peel, otherwise would damage cells.
Glycerin should be used in order to prevent drying of the peel
Coverslip needs to be placed in such a way that air bubbles are avoided
Blotting paper can be used to remove excess stain
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