Question

Two DNA , A and B have melting temperature (Tm) 340 and 350 k respectively .Can you draw any conclusion from these data regarding their base content ?

Answer 1

Hello !
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We Know that CG base pair has three H bonds and AT base pair has two H- bonds , therefore , CG base pair is more stable than AT base pair . Since sample B has higher melting temperature than sample A , therefore , Sample B has higher CG content as compared to sample A.
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Answer 2

the  sample of B of having higher melting temperature must be having more GC content as compared to sample A, since GC pairs having hydrogen bonds as compared to AT base pair which have only two hydrogen bonds.this result in stronger bonding in sample B