Question

Two proteins have same molecular weight and same isoelectric point what is the best way to resolve them

Answer 1

Answer:

Ion exchange chromatogrphy columns bind proteins with a negative net-charge. You could affect the net-charge of your protein by vary the pH of your buffer. At a pH above its isoelectric point, your protein has a negative net charge and will bind to an anion exchanger.

I think a Q-sepharose column has a better resolution but nevertheless you can try a DEAE sepharose as well. With a longer gradient (~20 cv) you have a better chance to separate both proteins.

Good luck

Answer 2

Answer:

Ion exchange chromatogrphy columns bind proteins with a negative net-charge. You could affect the net-charge of your protein by vary the pH of your buffer. At a pH above its isoelectric point, your protein has a negative net charge and will bind to an anion exchanger.

I think a Q-sepharose column has a better resolution but nevertheless you can try a DEAE sepharose as well. With a longer gradient (~20 cv) you have a better chance to separate both proteins.

Good luck