under what circumstances separation would be better by centrifugation method
Answers
Answer:
Which factors have an influence on centrifugation :
- Density of both samples and solution
- Temperature/viscosity
- Distance of particles displacement
- Rotation speed
Explanation:
Centrifugation is a technique used for the separation of particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed. The particles are suspended in a liquid medium and placed in a centrifuge tube. The tube is then placed in a rotor and spun at a define speed. Separation through sedimentation could be done naturally with the earth gravity, nevertheless, it would take ages. Centrifugation is making that natural process much faster. Rotation of the rotor about a central axis generates a centrifugal force upon the particles in the suspension.
A centrifuge is a device that separates particles from a solution through use of a rotor. In biology, the particles are usually cells, subcellular organelles, or large molecules, all of which are referred to here as particles. There are two types of centrifuge procedures; one is preparative, the purpose of which is to isolate specific particles, and the other is analytical, which involves measuring physical properties of the sedimenting particles. As a rotor spins in a centrifuge, a centrifugal force is applied to each particle in the sample; the particle will then sediment at the rate that is proportional to the centrifugal force applied to it. The viscosity of the sample solution and the physical properties of the particles also affect the sedimentation rate of each particle. At a fixed centrifugal force and liquid viscosity, the sedimentation rate of a particle is proportional to its size (molecular weight) and to the difference between the particle density and the density of the solution.
You can also refer to the image of a blood sample and its centrifugated sample....
Answer:
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