Use of fluorescence in the detection of molecules
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some cases, when the light energy is absorbed by a molecule, it raises some of the electrons to an excited state. ... Fluorescence is much less common than UV absorbance and because for a given molecule, both the excitation and emission wavelengths are specific, fluorescence detectors can be very selective.
Fluorescence is used mainly for measuring compounds in solution. ... In fluorescence spectrometry both an excitation spectrum (the light that is absorbed by the sample) and/or an emission spectrum (the light emitted by the sample) can be measured.
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