‘‘Use of heavy isotope of nitrogen by Meselson and Stahl demonstrated semi-conservative mode of replication of a DNA molecule.’’ Explain how did they arrive at this conclusion.
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Answer:
Use of heavy isotope by meson and stahl in their experiments as it could be easily distinguished from normal DNA.
Explanation:
• meson and stahl grew E. Coli in a medium with isotopic nitrogen as it's only source for many generations
• as a result the heavy isotopic nitrogen was incorporated in newly synthesied DNA and could be distinguished from normal DNA by centrigugation.
• they then transfered the medium with normal nitrogen and took samples at a definite time interval DNA extracted after 40 minutes was composed of equal amouth of hybrid and normal DNA
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