Question

vectors for cloning genes in plant and animals

Answer 1

Answer

• Tumour inducing plasmid (Ti) of soil bacterium.

• Agrobacterium tumefaciens has been made non virylent by deleting the part that is responsible for its virulence.

• However the modified plasmid does contain the tumour inducing gene T-DNA.

• It is how ever employed as recognition or cloning site.

• A foreign gene is inserted in T-DNA region of the plasmid.

• The T gene becomes inactive.

• The recombined or cloning vector is now passed into plasmid free bacterium.

• It is allowed to infect the plant or single cells in tissue culture.