Vicky'father wants to grow a single plant having 2 desirable characters colourful flowers andbig fruits.what method will you suggest him and why?
Answers
Answer:
Aim: To observe the different stages of Mitosis in Onion root tip.
Apparatus: Side, Microscope, Cover Slip, Dilute Hcl, Spirit Lamp, Glass Rod, Needle, Brush, Fresh Onion roots.
Procedure:
(i) Collect freshly grown Onion root itps, cut off the last 5mm of it and place them in a test tube.
(ii) Add Dilute Hcl and heat it on spirit lamp for 5 min. to soften the root tissue.
(iii) Decant the liquid carefully, wash the root tip on a watch glass containing water to remove Acid.
(iv) Remove excess water with the help of filter paper.
(v) Place the tip on the slide and add one drop of Methylated Blue.
(vi) Now, gently tap the root with end of the glass rod until it is completely broken.
(vii) Place a cover slip on tip over the warm gently for a few seconds over very low flame. Take care that it must not boil.
(viii) Put a piece of filter paper over the preparation and press the cover slip with your thumb avoiding any slide way movements.