Question

Volume of acd solution used to collect 100 ml of blood

Answer 1

• 15ml of Acid Citrate Dextrose (ACD) solution is required in order to preserve 100ml of blood.
• pH of ACD is maintained at 5.0
• ACD helps to prevent coagulation (blood clot formation) and haemolysis of blood (breaking down of red blood corpuscles)
• Citrate phosphate Dextrose solution (CPD) is another preservative that is used similar to ACD

Answer 2

Full form of ACD is Acid Citrate Dexrose. It's an anti-coagulant which is used in preservation of blood samples collected from subjects.

Blood cells tends to coagulate in absence of natural anti coagulants when taken out from the body of the subject. Thus the individual properties of blood cells get lost and the aim of the blood collection can not be fulfilled. Thus to keep the blood sample in a steady stable state anticoagulants like ACD and CPD( Citrate Phosphate Dextrose) is used[with a constant pH of 5 which is acidic than the blood pH itself, 7.2].

A standard volume of ACD solution used to preserve 100 ml of blood is 15 ml.