Question

What are cloning vectors ?write the main characteristics of any three types of cloning?

Answer 1

Characteristics of a cloning vectors

   it must be small in size

   It must be self-replicating inside host cell

   It must possess restriction site for Restriction Endonuclease enzymes

   Introduction of donor DNA fragment must not interfere with replication property of the vector

   It must possess some marker gene such that it can be used for later identification of recombinant cell

   it must possess multiple cloning site

Answer 2

Explanation:

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The different types of vectors available for cloning are plasmids, bacteriophages, bacterial artificial chromosomes (BACs), yeast artificial chromosomes (YACs) and mammalian artificial chromosomes (MACs). The cloning vectors are limited to the size of insert that they can carry

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