What are different carbon sources used in fermentation?
Answers
The technique of high pH anion-exchange chromatography method with pulsed amperometric detection (HPAEC-PAD) has been adapted for the rapid determination of common carbohydrates present in fermentation broths. Simple water dilution of filtered fermentation broth samples was the sole sample preparation step required. The samples were analyzed using a Dionex CarboPac PA1 column with 150 mM NaOH as the mobile phase at a flow-rate of 1 ml/min and a total run time of 20 min. A gradient method was also developed to resolve species which exhibited similar tR in the isocratic procedure. Among the analytes examined in this study were ethanol, glycerol, galactose, glucose, mannose, fructose, raffinose, ribose and lactose. Examples from several microbial fermentations using chemically defined or complex medium are presented.