Question

what are the 3 limitations of tissue culture

Answer 1

Answer:

The new plantlets can be grown in a short amount of time.Only a small amount of initial plant tissue is required.The new plantlets and plants are more likely to be free of viruses and diseases.The process is not dependant on the seasons and can be done throughout the year.You need only a relatively small space to perform the process (ten times the plants in one-tenth of the space).On a larger scale, the tissue culture process helps to supply the consumer market with new subspecies and variety.People looking to cultivate challenging plants such as specific breeds of orchid find more success with the tissue culture process than traditional soil.

Explanation:

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Answer 2

Answer:

1) Tissue Culture can require more labor and cost more money. There is a chance that the propagated plants will be less resilient to diseases due to the type of environment they are grown in.

2) Maintaining the sterile aseptic condition is the most difficult part of cell culture. Ø Chances of chemical and microbial contamination are very high in in vitro methods. Ø High possibility of cross contamination of different types of cells in culture.

3) It may be vulnerable to many environmental factors since they have same genetic material.

Explanation:

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