what are the 5 ligation product from the ligation reaction between the amplified GADPH gene and the blunted pJet 1.2 vector ? ( 200 word)
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Sequence of DNA bases that makes up the GAPDH gene that was cloned. Once the ... electrophoresis, restriction enzyme digestion, working with cloning vectors, blunt-end ... 5**. 6: Transformation. Transform bacteria with ligated product and plate them. 1 hr ... In other words, if the wrong fragment was amplified with the first.
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