What are the advantages and disadvantages of having cells like animal cells
Answers
Question:
What are the advantages and disadvantages of having cells like animal cells?
Answer:
Daniel, just to add to what's been said, here are some important questions to consider.
1. What amount of protein do you need, and for what purpose? Is your aim to grow a crystal, or perform biochemical characterization, or to raise an antibody?
2. What is the species your protein of interest originated from and what systems have people working on the same or related proteins successfully used to express it? Certain eukaryotic proteins will not fold correctly in bacteria for example, while S. cerevisiae will frequently glycosylate proteins post-translationally, which may not be desirable for your purposes or may harm the yield. An insect or mammalian cell expression system may give you protein that is closest in form to what you are studying, if it originated from humans or mice.
3. What can you afford to use in your lab? Bacteria are cheap to culture in large amounts but if you have to go with a mammalian cell system, it may prove to be fairly expensive.
Explanation:
Hope this answer will help you.✌️