Question

what are the principles of Biurat's test , ninhydrin test and millon's test of protiens?

Answer 1

Answer:

Biuret Test:

The Biuret test for proteins qualitatively detects the

presence of proteins in solution with the development

of a deep violet colour. In alkaline condition, Biuret

(NH2-CO-NH-CO-NH2) reacts with compounds

containing two or more peptide bonds to form

complexes of violet colour.

Answer 2

Answer:

Biuret Test:

The Biuret test for proteins qualitatively detects the presence of proteins in solution with the development of a deep violet colour. In alkaline condition, Biuret (NH2-CO-NH-CO-NH2) reacts with compounds containing two or more peptide bonds to form complexes of violet colour.