What are the steps involved to test that photosynthesis has taken place or not in a plant.
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It is convenient to divide the photosynthetic process in plants into four stages, each occurring in a defined area of the chloroplast: (1) absorption of light, (2) electron transport leading to the reduction of NADP+ to NADPH, (3) generation of ATP, and (4) conversion of CO2 into carbohydrates (carbon fixation).
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heat a plant leaf in boiling water for 30 seconds (this kills the leaf, stopping any chemical reactions) add the leaf to boiling ethanol in a water bath for a few minutes (the boiling ethanol dissolves the chlorophyll and removes the green colour from the leaf - it turns white so it is easy to see the change in colour)
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