What do you mean by optimum temperature, pH and denaturation of enzyme ?
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When enzymes denature, they are no longer active and cannot function. Extreme temperature and the wrong levels of pH -- a measure of a substance's acidity or alkalinity -- can cause enzymes to become denatured..
The optimal pH of an enzyme is the pH at which it is most active. Enzymes can be thought of as biological catalysts that control many physiology related chemical reactions in the body of an organism. An enzyme will work most efficiently at or near its optimum pH. ... This peak represents the optimum pH...
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