What does fluorescence spectroscopy measure?
Answers
Fluorescence is the phenomenon where a molecule absorbs light within its absorption band and then emits this light at longer wavelengths within its emission band. This phenomenon can be used to identify, quantify, and observe chemical activity, and it is a popular method due to its high levels of sensitivity, simplicity, and specificity.
Fluorescence spectroscopy is a spectroscopy method used to analyze the fluorescence properties of a sample by determining the concentration of an analyte in a sample. This technique is widely used for measuring compounds in a solution, and it is a relatively easy method to perform
What does fluorescence spectroscopy measure?
the instrument uesd to measure fluorescence is called a fluorometer or
fluorimeter.a fluorometer generate the wavelength of light required to
excite the analyte of interest .it selectively transmits the wavelength of light
emitted then measures the intensity of the emitted light
Generally molecules that fluoresce are conjugated systems.Fluorescence occure when an atom or molecules relax through vibrational relaxation to
its ground state after being electricaly excited .the specific frequencies of excitation and emission are dependent on the molecule or atom.
Fluorescence spectroscopy (known as fluorimetry or spectrofluorometry) is
a type of electromagnetic spectroscopy that analyzes fluorescence from a sample .its involve using a beam light usually ultraviolet light that excites
the electrons in molecules of certain compounds and causes them to emit light typically but not necessarily visible light .a complementary technique
is absorption spectroscopy .in the special case of single molecule fluorescence spectroscopy intensity fluctuation from the emitted light are
measured from either single fluorophores or pairs of fluorophores.
devices that measure fluorscence sre called fluorometers