Question

What happenes if there is continuous derivation of cell line

Answer 1

Answer:

• Most of the cell cultures used in the diagnostic laboratories are continuous cells lines.
• Continuous immortalized cell lines are comprised of a single cell type that can be serially propagated in culture either for a limited number of cell divisions (approximately thirty) or otherwise indefinitely.
• Cell lines of a finite life are usually diploid and maintain some degree of differentiation.
• The specimen is introduced into the cell culture medium and the presence of viruses is detected by observing the cells for a cytopathic effect (CPE).
• Cell culture techniques are specialized and labor intensive and some viruses either causes no cytopathic effects or cause a CPE that takes a week or more to evolve.

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