Question

What is immunotaxonomy? Discuss the principles and
and techniques for
immunodiffusion and radioimmunoassay in immunotaxonomy.
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Answer 1

Explanation:

• Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar which is generally soft gel agar (2%) or agarose (2%), used for the detection of antibodies or antigen. The commonly known types are 1. ... Single diffusion in two dimension (radial immunodiffusion or Mancini method) 4.

PRINCIPLE  Radioimmunoassay (RIA)

• involves the separation of a protein (from a mixture) using the specificity of antibody - antigen binding and quantitation using radioactivity. ...  At increasing concentrations of unlabeled antigen, an increasing amount of radioactive antigen is displaced from the antibody molecules.

Principle of Radial Immunodiffusion

• Circular wells are cut into the agar and antigen is introduced into the wells. Specific antigens to the impregnated antibodies diffuse through the agar in all directions from the well and react with the antibody present forming visible precipitate or a precipitin ring.

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Answer 2

Answer:

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