Question

what is killing and fixation in botany​

Answer 1

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Answer 2

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discussion::Fixation of tissues is the most crucial step in the preparation of tissue for observation in the transmission electron microscope.

The goal of fixation is to preserve structure as faithfully as possible compared to the living state.

important parameters to remember about fixation are: (1) keep the time between killing and fixation to a minimum. (2) keep the size of the tissue as small as possible without losing information or destroying the tissue. If a large specimen must be fixed, keep one dimension less than 1 mm, or else nick areas of the specimen that can be discarded so that the fixative can penetrate. Effective penetration of fixative is about 0.5 mm for osmium.

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