what is mounting in chapter microorganisms
Answers
1. A slide is cleaned properly under tap water, such that water does not remain as drops on its surface2. The slide is dried by wiping with bibulous paper and subsequently moving over flame or keeping in the sun.
3. If the bacteria has been grown on a solid medium (as slant culture or plate culture), a drop of distilled water is put at the center of the slide. A loop is sterilized by heating it over a flame to red hot. It is cooled to room temperature. Then, aseptically a small portion of bacteria culture is scraped by the loop and transferred to the drop of water.
A suspension of bacteria is made by gently mixing the loop of bacteria in the water drop, in such a way that, the drop does not spread. If grown in a liquid medium as suspension, aseptically a drop of the bacteria suspension is placed at the center of the slide directly, using a flame-sterilized loop.
4. Petroleum jelly or similar material is applied surrounding the drop, so that, when the cover slip covers the drop, evaporation and effect of air current is minimized.
5. A cover slip is kept in a slanting position near the drop of bacteria suspension with one edge touching the slide.
6. Slowly the cover slip is lowered, till it touches the surface of the drop.
7. Then, the cover slip is released, so that the drop of bacteria suspension spreads in all directions under the cover slip. Care should be taken, so that no air bubble is formed in the suspension.
1. A slide is cleaned properly under tap water, such that water does not remain as drops on its surface2. The slide is dried by wiping with bibulous paper and subsequently moving over flame or keeping in the sun.
3. If the bacteria has been grown on a solid medium (as slant culture or plate culture), a drop of distilled water is put at the center of the slide. A loop is sterilized by heating it over a flame to red hot. It is cooled to room temperature. Then, aseptically a small portion of bacteria culture is scraped by the loop and transferred to the drop of water.
A suspension of bacteria is made by gently mixing the loop of bacteria in the water drop, in such a way that, the drop does not spread. If grown in a liquid medium as suspension, aseptically a drop of the bacteria suspension is placed at the center of the slide directly, using a flame-sterilized loop.
4. Petroleum jelly or similar material is applied surrounding the drop, so that, when the cover slip covers the drop, evaporation and effect of air current is minimized.
5. A cover slip is kept in a slanting position near the drop of bacteria suspension with one edge touching the slide.
6. Slowly the cover slip is lowered, till it touches the surface of the drop.
7. Then, the cover slip is released, so that the drop of bacteria suspension spreads in all directions under the cover slip. Care should be taken, so that no air bubble is formed in the suspension
Read more on Brainly.in - https://brainly.in/question/8637032#readmore