Question

What is polymerized form of horseradish peroxidase

Answer 1

The mechanism of the horseradish peroxidase (HRP)–H2O2-catalysed polymerisation of aniline in the presence of AOT vesicles was investigated. AOT (= bis-(2-ethylhexyl)sulfosuccinate) served as vesicle-forming surfactant and dopant for obtaining at pH = 4.3 and room temperature within 24 h under optimal reaction conditions the green emeraldine salt form of polyaniline in 90–95% yield. Based on UV/VIS/NIR and EPR measurements carried out during the polymerisation reaction, and based on changes in aniline and H2O2 concentrations and HRP activity, a mechanism is proposed.

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