Question

What is principle of thin layer chromatography?

Answer 1

Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two phases between which they are to be partitioned. Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase).

Answer 2

It is defined as the process of separation of the individual components of a mixture based on their relative affinities towards stationary and mobile phases. Principle: The samples are subjected to flow by mobile liquid onto or through the stable stationary phase.

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