what is role of glycerol is used in preparation of competent cells ?
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Glycerol prevents the formation of ice crystals when aqueous solutions are frozen. The ice crystals will damage the cellmembranes, killing the cells - so preventing ice crystals is the key to cryopreservation. its a cryopretectant, some ppl woulduse DMSO for mammalian cells.
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A cryoprotectant with a higher freezing point than water. It protects the bacterial cells from the formation of ice crystals during -80 C freezing and storage. Glycerol excludes water from the space between cells and facilitates quicker freezing with its higher freezing point.
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